Mon Jun 8, 2015 4:38 pm (PDT) . Posted by:
"Rick Laferriere" ri_lymeinfo
*Changes in antibody reactivity to /Borrelia burgdorferi/
three months after a tick bite. A cohort of 1,886 persons. *
Dessau RB, Fryland L, Wilhelmsson P, Ekerfelt C, Nyman D,
Forsberg P, Lindgren PE
Clinical and Vaccine Immunology, pii: CVI.00026-15. Online
first, 2015 May 20.
http://doi.org/10.1128/CVI.00026-15
<http://doi.org/10.1128/CVI.00026-15>
Abstract
Lyme borreliosis is a tick-borne disease caused by the
bacterium Borrelia burgdorferi. The most frequent clinical
manifestation is a rash called erythema migrans. Changes in
antibody reactivity to B. burgdorferi three months after a
tick bite are measured using ELISA assays.
One assay is based on native purified flagella-antigen (IgG)
and the other assay is based on a recombinant antigen called
C6 (IgG or IgM). Paired samples were taken at the time of a
tick bite and three months later from 1,886 persons in
Sweden and the Åland Islands. The seroconversion or relative
change is defined as the measurement units from the second
sample divided by the first sample. Thresholds for the
minimum level of significant change were defined as the 2.5%
percentile to represent the random error level. The
thresholds were 2.7 fold rises for the flagella IgG and 1.8
fold rises for the C6 assays.
102/101 (5.4%) of 1,886 persons had a significant rise in
antibody reactivity in the flagella or C6 assay. Among 40
cases with a diagnosis of Lyme borreliosis the sensitivity
of a rise in antibodies was 33% and 50% to the flagella
antigen and the C6 antigen respectively. Graphical methods
to display the antibody response and methods to choose
thresholds for a rise in relative antibody reactivity are
shown and discussed.
In conclusion, 5.4% of people with tick bites developed a
rise in borrelia-specific antibodies above the 2.5%
percentile in either ELISA assay, but only 40 (2.1%)
developed clinical Lyme borreliosis.
http://doi.org/10.1128/CVI.00026-15
three months after a tick bite. A cohort of 1,886 persons. *
Dessau RB, Fryland L, Wilhelmsson P, Ekerfelt C, Nyman D,
Forsberg P, Lindgren PE
Clinical and Vaccine Immunology, pii: CVI.00026-15. Online
first, 2015 May 20.
http://doi.org/10.1128/CVI.00026-15
<http://doi.org/10.1128/CVI.00026-15>
Abstract
Lyme borreliosis is a tick-borne disease caused by the
bacterium Borrelia burgdorferi. The most frequent clinical
manifestation is a rash called erythema migrans. Changes in
antibody reactivity to B. burgdorferi three months after a
tick bite are measured using ELISA assays.
One assay is based on native purified flagella-antigen (IgG)
and the other assay is based on a recombinant antigen called
C6 (IgG or IgM). Paired samples were taken at the time of a
tick bite and three months later from 1,886 persons in
Sweden and the Åland Islands. The seroconversion or relative
change is defined as the measurement units from the second
sample divided by the first sample. Thresholds for the
minimum level of significant change were defined as the 2.5%
percentile to represent the random error level. The
thresholds were 2.7 fold rises for the flagella IgG and 1.8
fold rises for the C6 assays.
102/101 (5.4%) of 1,886 persons had a significant rise in
antibody reactivity in the flagella or C6 assay. Among 40
cases with a diagnosis of Lyme borreliosis the sensitivity
of a rise in antibodies was 33% and 50% to the flagella
antigen and the C6 antigen respectively. Graphical methods
to display the antibody response and methods to choose
thresholds for a rise in relative antibody reactivity are
shown and discussed.
In conclusion, 5.4% of people with tick bites developed a
rise in borrelia-specific antibodies above the 2.5%
percentile in either ELISA assay, but only 40 (2.1%)
developed clinical Lyme borreliosis.
http://doi.org/10.1128/CVI.00026-15
No comments:
Post a Comment
Please be constructive in your comments.